Avian adeno-associated virus as an anterograde transsynaptic vector

Retrograde and anterograde transsynaptic viral vectors are useful tools for studying the input output organization of neuronal circuitry, respectively. While retrograde widely used, that show transduction not common. We chose recombinant avian adeno-associated virus (A3V) carrying mCherry gene injected it into eyeball, cochlear duct, midbrain auditory center chickens. observed different survival times to examine transcellular transport resulting expression. To confirm mode, we co-injected A3V cholera toxin B subunit. Injecting eyeball cochlea labeled neurons in visual pathways, Second-, third-order labeling occurred approximately two seven days, respectively, after injection midbrain. The distribution strongly suggests is preferentially transduces postsynaptic neurons. displays no extrasynaptic leakage moderate speed synapse passage, which better than other viruses previously reported. Compared with AAV1&9, have been shown pass one anterogradely, passes several synapses direction. would be a good tool study topographic projection axons their target